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Image Search Results
Journal: Neuron
Article Title: Loss of nigral excitation of cholinergic interneurons contributes to Parkinsonian motor impairments
doi: 10.1016/j.neuron.2021.01.028
Figure Lengend Snippet: (A) Schematic of design and injection of AAV-DIO-mGluR1-FLAG in ChAT-Cre mice.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-TH Millipore Cat# AB152 Donkey anti-rabbit Alexa Fluor 488 Life Technologies Cat# A21206 Rabbit anti-DDDDK tag Abcam Cat# ab1162 Donkey anti-rabbit Alexa Fluor 594 Abcam Cat# ab150076 Bacterial and Virus Strains AAV5.EF1a.DIO.hChR2 (H134R)-EYFP pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA was a gift from Karl Deisseroth Addgene plasmid: 20298 AAV9.hSyn.tdTomato.T2A.mGIRK2-1 Marcott et al., 2014 Penn Vector Core: V3992MI-R AAV5-hSyn-DIO-hM3D-mCherry Krashes et al., 2011 Addgene plasmid: 44361
Techniques: Injection
Journal: Neuron
Article Title: Loss of nigral excitation of cholinergic interneurons contributes to Parkinsonian motor impairments
doi: 10.1016/j.neuron.2021.01.028
Figure Lengend Snippet: (A) Representative traces from DLS ChIs from saline (left), 6-OHDA (middle) and 6-OHDA + mGluR1 overexpressing (right) groups following photostimulation (5 flashes, 2 ms, 20 Hz) of midbrain inputs.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-TH Millipore Cat# AB152 Donkey anti-rabbit Alexa Fluor 488 Life Technologies Cat# A21206 Rabbit anti-DDDDK tag Abcam Cat# ab1162 Donkey anti-rabbit Alexa Fluor 594 Abcam Cat# ab150076 Bacterial and Virus Strains AAV5.EF1a.DIO.hChR2 (H134R)-EYFP pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA was a gift from Karl Deisseroth Addgene plasmid: 20298 AAV9.hSyn.tdTomato.T2A.mGIRK2-1 Marcott et al., 2014 Penn Vector Core: V3992MI-R AAV5-hSyn-DIO-hM3D-mCherry Krashes et al., 2011 Addgene plasmid: 44361
Techniques:
Journal: Neuron
Article Title: Loss of nigral excitation of cholinergic interneurons contributes to Parkinsonian motor impairments
doi: 10.1016/j.neuron.2021.01.028
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-TH Millipore Cat# AB152 Donkey anti-rabbit Alexa Fluor 488 Life Technologies Cat# A21206 Rabbit anti-DDDDK tag Abcam Cat# ab1162 Donkey anti-rabbit Alexa Fluor 594 Abcam Cat# ab150076 Bacterial and Virus Strains AAV5.EF1a.DIO.hChR2 (H134R)-EYFP pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA was a gift from Karl Deisseroth Addgene plasmid: 20298 AAV9.hSyn.tdTomato.T2A.mGIRK2-1 Marcott et al., 2014 Penn Vector Core: V3992MI-R AAV5-hSyn-DIO-hM3D-mCherry Krashes et al., 2011 Addgene plasmid: 44361
Techniques: Plasmid Preparation, Recombinant, Software